It is known that activation of the Gs G-protein coupled receptor (GPCR) pathway in osteoblasts can lead to significant increases in trabecular bone formation. However, the effects of constitutive Gs signaling on bone tissue quality are not known. Using Fourier transform infrared (FTIR) spectroscopy and synchrotron radiation μCT (SRμCT), we evaluate the bone composition of double transgenic mice that exhibit osteoblast-specific constitutive Gs signaling activity. Our findings demonstrate that Gs activity in osteoblasts leads to the deposition of immature bone tissue with reduced mineralization.